![]() Thanks to the holographic focusing flexibility and an ad-hoc angles’ tracking algorithm, we combine different phase-contrast views of the nuclei to retrieve their 3D refractive index distribution. In fact, the loss of water reduces the turgor pressure and we recognize it induces significant rotation of cells’ nuclei. ![]() Here we show an intriguing concept based on an inherent natural process for plants biology, i.e., dehydration, allowing us to easily obtain 3D-tomography of onion-epidermal cells’ nuclei. ![]() It involves probing cells with light at wide angles, which unfortunately requires complex systems. Single-cell phase-contrast tomography promises to become decisive for studying 3D intracellular structures in biology.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |